Cell Culture Media

[+] Earle's salts, L-glutamine Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells. When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts. This formulation contains Earle's salts and L-glutamine.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] Earle's salts, L-glutamine Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells. When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts. This formulation contains Earle's salts and L-glutamine.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] 4.5 g/L glucose, L-glutamine, sodium pyruvate Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-013-CV, -CM, -LB, -LX contain 4.5 g/L glucose, L-glutamine and sodium pyruvate.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] 4.5 g/L glucose, L-glutamine, sodium pyruvate Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-013-CV, -CM, -LB, -LX contain 4.5 g/L glucose, L-glutamine and sodium pyruvate.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] 4.5 g/L glucose, L-glutamine, sodium pyruvate Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-013-CV, -CM, -LB, -LX contain 4.5 g/L glucose, L-glutamine and sodium pyruvate.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] 1 g/L glucose, L-glutamine, sodium pyruvate Technical advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) that containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-014-CV and -CM contain 1.0 g/L glucose, L-glutamine, and sodium pyruvate.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] 1 g/L glucose, L-glutamine, sodium pyruvate Technical advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) that containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-014-CV and -CM contain 1.0 g/L glucose, L-glutamine, and sodium pyruvate.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine,25 mM HEPES[-] ß-thioglycerol, ß-mercaptoethanol Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Iscove's Modification of DMEM (IMDM) is a highly enriched synthetic medium optimized for rapidly proliferation cultures of high cell density. A modified form of Dulbecco's Modified eagle Medium (DMEM), Iscove's contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types. This formulation is produced with L-glutamine and 25 mM HEPES, and do not contain alpha-thioglycerol and beta-mercaptoethanol.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine, 25 mM HEPES[-] ß-thioglycerol, ß-mercaptoethanol Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Iscove's Modification of DMEM (IMDM) is a highly enriched synthetic medium optimized for rapidly proliferation cultures of high cell density. A modified form of Dulbecco's Modified eagle Medium (DMEM), Iscove's contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types. This formulation is produced with L-glutamine and 25 mM HEPES, and do not contain alpha-thioglycerol and beta-mercaptoethanol.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] 4.5 g/L glucose, L-glutamine[-] sodium pyruvate Technical Advantage: Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 10-017-CV and -CM contain L-glutamine and 4.5 g/L glucose and do not contain sodium pyruvate.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] Earle's salts, ribonucleosides, deoxyribonucleosides, L-glutamine with Earle's salts, ribonucleosides, deoxyribonucleosides, and L-glutamine.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine Technical Advantage: This formulation is not available from other major suppliers. A modified formulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

With L-glutamine A modification of Ham's F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original.  It is formulated to support the growth of cells including differentiated rat and chicken cell types and primary human hepatocytes.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine[-] phenol red A modified formulation of Minimum Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine Technical Advantage: Manufactured under industry-leading standards for low endotoxin content Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. This formulation is produced with L-glutamine.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. This formulation is produced with L-glutamine.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. This formulation is produced with L-glutamine.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine, 25 mM HEPES Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. This formulation includes L-glutamine and glucose.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine[-] glucose Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy’s 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. This formulation includes L-glutamine is does not have glucose.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

With L-glutamine Leibovitz's L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments.  It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine.  The formation of acid metabolite byproducts is prevented by replacing glucose with galactose and sodium pyruvate.  Examples of usage include the propagation of HEP-2, monkey kidney cells and primary explants of embryonic and adult human tissue.  Leibovitz's L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

[+] L-glutamine McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. Originally modified by Iwakata and Grace, this version of McCoy's 5A includes increased levels of inositol and glucose.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.