Growth Medium

Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 5 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogensTested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay.

Source

EHS mouse tumor

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.

Corning® Endothelial Cell Growth Supplement (ECGS), 100mg, is a broadly used supplement to culture a variety of cells, particularly endothelial cells. ECGS contains various growth factors (e.g., acidic FGF or ECGF-a). 1/pack

Formulation

Lyophilized from NaCl-containing buffer with streptomycin sulfate; reconstitute in serum-free medium

For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.

Source

Bovine brain

Quality

• Tested for ability to promote proliferation of Fetal Bovine Heart Endothelial Cells (FBHEC) in medium containing 10% newborn calf serum
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Preparation and Storage

Stable for at least three months at 2° to 8°C (lyophilized) or = one month at -20°C (solubilized). Avoid multiple freeze/thaws.

Tip

Recommended concentration: 50 to 150 µg/ mL of medium depending on cell type. Some primary cells require supplementation of 1 to 100 µg/ mL heparin.

Corning® Nu-Serum™ IV Growth Medium Supplement, 500 mL, is a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture. 1/pack, formerly Cat. #55004

Tip

Replaces fetal bovine and other sera on an equivalent volume basis

Quality

• Tested at a concentration of 10% for the ability to stimulate a = 100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells
• Tested for total protein content, pH, and osmolarity (information included with product)
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption
• Tested for endotoxin (LAL assay)

Storage

Shelf life: >= 3 months from date of shipment at -20°C

Corning® Nu-Serum™ Growth Medium Supplement, 500 mL, is a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture. 1/pack, formerly Cat. #55000

Preparation and Storage

Stable for at least three months at -20°C

Tip

Replaces fetal bovine and other sera on an equivalent volume basis

Quality

• Tested at a concentration of 10% for the ability to stimulate a = 100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells
• Tested for total protein content, pH, and osmolarity (information included with product)
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption
• Tested for endotoxin (LAL assay)

Storage

Shelf life: >= 3 months from date of shipment at -20°C

Corning® Nu-Serum™ IV Growth Medium Supplement, 100 mL, is a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture. 1/pack, formerly Cat. #51004

Preparation and Storage

Stable for at least three months at -20°C

Tip

Replaces fetal bovine and other sera on an equivalent volume basis

Quality

• Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells
• Tested for total protein content, pH, and osmolarity (information included with product)
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption
• Tested for endotoxin (LAL assay)

Formulation

Corning Nu-Serum™ IV - Frozen solution containing 25% fetal bovine serum, EGF, ECGS, insulin, human transferrin, triiodothyronine, progesterone, estradiol-17ß, testosterone, hydrocortisone, selenous acid, o-phosphorylethanolamine, glucose, amino acids, vitamins, and other trace elements and nutrients contained in the Ham's F12 medium base.

Storage

Shelf life: >= 3 months from date of shipment at -20°C

Corning® Nu-Serum™ Growth Medium Supplement, 100 mL, provides a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture. 1/pack, formerly Cat. #51000

Tip

Replaces fetal bovine and other sera on an equivalent volume basis

Quality

• Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a =13-fold increase in growth of BALB/c-3T3 cells
• Tested for total protein content, pH, and osmolarity (information included with product)
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption
• Tested for endotoxin (LAL assay)

Storage

Shelf life: >= 3 months from date of shipment at -20°C

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Source

Engelbreth-Holm-Swarm Mouse Tumor

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing performed to screen for a number of pathogensTested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Gel stability tested for a period of 14 days at 37°C
• Biological activity determined for each lot using neurite outgrowth assay.

Source

EHS mouse tumor

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.

Corning® basic Fibroblast Growth Factor (bFGF), human recombinant, 10µg, is a heparin-binding mitogenic protein that enhances the proliferation of a wide variety of cell types under serum-free or serum-reduced conditions. 1/pack, formerly Cat. #40060. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

Lyophilized from phosphate buffered saline, carrier-free; reconstitute in dH2O or serum-free medium

Preparation and Storage

Stable for at least three months at -20°C (lyophilized) or = one month solubilized at -20°C. Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality

• Purity: >95% by SDS-PAGE
• Tested for ability to promote proliferation of FBHEC
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
• Recombinant, E. coli

Molecular Weight

17 kD

Tip

Recommended concentration: 1 to 20 ng/ mL of medium depending on cell type

Storage

• Storage: at least three months at 2-8°C (lyophilized) or one month at -20°C (solubilized)
• Shelf life: >= 3 months from date of shipmen

Corning® Epidermal Growth Factor (EGF), human recombinant, 100µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 1/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

Lyophilized from acetic acid, carrier-free; reconstitute in dH2O or aqueous buffer

Preparation and Storage

Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality

• Purity: >98% by SDS-PAGE and reverse phase HPLC
• Shown to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Source

Recombinant, E. coli

Molecular Weight

6.2 kD

Tip

Recommended concentration: 0.1 to 1.0 ng/ mL medium depending on cell type

Corning® Transforming Growth Factor-beta (TGF-beta), human natural, 1µg, is a multifunctional protein that plays a central role in the regulation of cell growth and differentiation with either stimulatory or inhibitory effects. 1/pack, formerly Cat #40039 These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

Frozen in 36% acetonitrile-0.1% trifluoroacetic acid.

Caution: TGF-ß absorbs to glass and plastic surfaces (instructions for dilution are provided with the product)

Source

• Human platelets. NOTE: Source material tested for hepatitis B antigen and HIV-1 antibody
• Molecular Weight: 25 kD

Quality

• Purity: >95% by SDS-PAGE
• Tested for ability to inhibit DNA synthesis of Mv 1Lu mink lung cells (ATCC CCL-64)
• Tested and found negative for bacteria, fungi and mycoplasma

Tip

Recommended concentration: 10-50 pg/ mL of medium depending on cell type and application

Preparation and Storage

Stable for at least three months at -70°C (if diluted according to instructions)

Storage

Shelf life: >= 3 months from date of shipment at -70°C

Corning® Epidermal Growth Factor (EGF), mouse natural (receptor grade), 100µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 1/pack These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O

Preparation and Storage

Stable for at least three months at 2° to 8°C (lyophilized) or = one month solubilized at -20°C . Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality

• Purity: >98% by SDS-PAGE and pH 9.9 PAGE
• Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma

Source

Male mouse submaxillary glands

Molecular Weight

6.1 kD

Purification

Purified by BioGel® P10/DEAE chromatography

Tip

Recommended concentration: Approximately 5 ng/ mL medium depending on cell type

Storage

Shelf life: >= 3 months from date of shipment at 2-8°C (lyophilized) or >= one month at -20°C (solubilized)

Corning® Endothelial Cell Growth Supplement (ECGS), 15mg, is a broadly used supplement to culture a variety of cells, particularly endothelial cells. ECGS contains various growth factors (e.g., acidic FGF or ECGF-a). 1/pack

Source

Bovine brain

Quality

• Tested for ability to promote proliferation of Fetal Bovine Heart Endothelial Cells (FBHEC) in medium containing 10% newborn calf serum
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Preparation and Storage

Stable for at least three months at 2° to 8°C (lyophilized) or = one month at -20°C (solubilized). Avoid multiple freeze thaws.

Tip

Recommended concentration - 50-150 µg/ mL of medium depending on cell type. Some primary cells require supplementation of 1-100 µg/ mL heparin.

Formulation

Lyophilized from NaCl-containing buffer with streptomycin sulfate; reconstitute in serum-free medium.

Storage

Shelf life: >= 3 months from date of shipment at 2-8°C (lyophilized) or >= one month at -20°C (solubilized)