Ingredients per litre
Tryptone : 10 g
Sodium Chloride : 10 g
Yeast extract : 5 g
store dry at room temperature
dissolve 25 g in 1 l distilled water and adjust the pH to 7.2.
sterilize by autoclaving at 121°C for 15 minutes.
H. Miller, Propagation and maintenance of E. coli for the preparation of
phage and plasmid DNA. , Meths. Enzymol. 152, 145 (1987)
S. Heber, B.E. Tropp, Biochim. Biophys. Acta 1129, 1 (1991)